Recombinant Dna Technology

Author: Sardul Singh Sandhu
Publisher: I. K. International Pvt Ltd
ISBN: 9789380578446
Size: 19.37 MB
Format: PDF, Docs
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The objective of the book is to introduce the basic principle and techniques used to make Recombinant DNA. The book commences with an introduction to different tools used for Gene cloning. The final chapters cover the application of Recombinant Technology on current research and provide an inside look on Human Genome Project, Ribozyme Technology, Antisense technology, DNA sequencing, Protein Engineering, Transgenic technology and development of vaccines. It features summary of chapter in the form of flow charts, highlighting the key points. The book also includes an appendix which provides in depth descriptions of protocols which cover the basic aspects of Molecular biology and glossary defining nearly all the possible terms mentioned in the book. The purpose of this book is to provide an insight on theoretical aspects of Recombinant DNA manipulation with special emphasis on different procedures to create chimeric molecules using examples from actual experimental works. The book has been designed for under-graduates, post-graduates and technicians who wish to know and use the principles and techniques of Recombinant DNA Technology

Recombinant Dna

Author: James D. Watson
Publisher: Macmillan
ISBN: 0716722828
Size: 19.73 MB
Format: PDF, Docs
View: 90

An overview of recombitant DNA techniques and surveys advances in recombinant molecular genetics, experimental methods and their results.

Purification And Analysis Of Recombinant Proteins

Author: Ramnath Seetharam
Publisher: CRC Press
ISBN: 0824782771
Size: 14.15 MB
Format: PDF, Docs
View: 47

Covering both new and traditional topics in the purification and analysis of recombinant proteins, this volume demonstrates how to overcome problems in protein research and presents practical methods used in protein work, explaining their theoretical bases. The collection also explores innovative co

Recombinant Protein Drugs

Author: P. Buckel
Publisher: Springer Science & Business Media
ISBN: 3764359048
Size: 13.43 MB
Format: PDF, Mobi
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Recombinant protein drugs are intimately associated with the impressive success story of the Biotech Industry during the past thirty years, some of them belonging to the most successful pharmaceutical products. More than thirty different proteins are available for a variety of clinical applications, over 300 proteins are presently being evaluated in clinical trials. In this new volume of the MDT series, historical, technical and clinical aspects of recombinant protein drug discovery and development are presented, covering past, present and future highlights. Leading scientists and co-founders of early Biotech companies describe technical breakthroughs and the fascinating story of pioneering discoveries, as well as the long way of translating them into products and business. Therefore, this book represents an exciting documentation of the beginning of a new era in the pharmaceutical industry. In addition, scientists from basic research, clinic and industry actively involved in new developments discuss...

Manipulation And Expression Of Recombinant Dna

Author: Sue Carson
Publisher: Academic Press
ISBN: 9780080456546
Size: 15.29 MB
Format: PDF, ePub, Docs
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This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein. The second edition has been completely re-written, with new laboratory exercises and all new illustrations and text, designed for a typical 15-week semester, rather than a 4-week intensive course. The “project approach to experiments was maintained: students still follow a cloning project through to completion, culminating in the purification of recombinant protein. It takes advantage of the enhanced green fluorescent protein—students can actually visualize positive clones following IPTG induction. *Cover basic concepts and techniques used in molecular biology research labs *Student-tested labs proven successful in a real classroom laboratories *Exercises simulate a cloning project that would be performed in a real research lab *"Project" approach to experiments gives students an overview of the entire process *Prep-list appendix contains necessary recipes and catalog numbers, providing staff with detailed instructions