Recombinant Dna Technology

Author: Sardul Singh Sandhu
Publisher: I. K. International Pvt Ltd
ISBN: 9789380578446
Size: 15.81 MB
Format: PDF, ePub, Docs
View: 64

The objective of the book is to introduce the basic principle and techniques used to make Recombinant DNA. The book commences with an introduction to different tools used for Gene cloning. The final chapters cover the application of Recombinant Technology on current research and provide an inside look on Human Genome Project, Ribozyme Technology, Antisense technology, DNA sequencing, Protein Engineering, Transgenic technology and development of vaccines. It features summary of chapter in the form of flow charts, highlighting the key points. The book also includes an appendix which provides in depth descriptions of protocols which cover the basic aspects of Molecular biology and glossary defining nearly all the possible terms mentioned in the book. The purpose of this book is to provide an insight on theoretical aspects of Recombinant DNA manipulation with special emphasis on different procedures to create chimeric molecules using examples from actual experimental works. The book has been designed for under-graduates, post-graduates and technicians who wish to know and use the principles and techniques of Recombinant DNA Technology

Recombinant Dna

Author: James D. Watson
Publisher: Macmillan
ISBN: 0716722828
Size: 18.93 MB
Format: PDF, ePub
View: 42

An overview of recombitant DNA techniques and surveys advances in recombinant molecular genetics, experimental methods and their results.

Recombinant Dna Technology

Author: Keya Chaudhuri
Publisher: The Energy and Resources Institute (TERI)
ISBN: 9788179933206
Size: 20.49 MB
Format: PDF, Docs
View: 35

Recombinant DNA Technology is focussed on the current state of knowledge on the recombinant DNA technology and its applications. The book will provide comprehensive knowledge on the principles and concepts of recombinant DNA technology or genetic engineering, protein expression of cloned genes, PCR amplification of DNA, RFLP, AFLP and DNA fingerprinting and finally the most recent siRNA technology. It can be used by post-graduate students studying and teachers teaching in the area of Molecular Biology, Biotechnology, Genetics, Microbiology, Life Science, Pharmacy, Agriculture and Basic Medical Sciences.

Recombinant Dna Genes And Genomes

Author: James D. Watson
Publisher: Macmillan
ISBN: 0716728664
Size: 10.13 MB
Format: PDF, Docs
View: 55

This text presents an introduction to the concepts and techniques of recombinant DNA research and their dramatic results. It explores core concepts in molecular biology in a contemporary inquiry-based context, building its coverage around the landmark experiments that redefined our understanding of DNA.

Recombinant Dna Principles And Methodologies

Author: James Greene
Publisher: CRC Press
ISBN: 0824799895
Size: 15.10 MB
Format: PDF, Docs
View: 33

This comprehensive yet balanced work emphasizes the principles and rationale underlying recombinant DNA methodology while furnishing a general understanding of the experimental protocols-suggesting flexible approaches to resolving particular molecular necessities that are easily adaptable to readers' specific applications. Features summary tables presenting at-a-glance information on practices of recombinant DNA methodologies! Recombinant DNA Principles and Methodologies discusses basic and advanced topics requisite to the employment of recombinant DNA technology, such as plasmid biology nucleic acid biochemistry restriction enzymes cloning strategies gel electrophoresis southern and northern blotting preparation of probes phage lambda biology cosmids and genome analysis cloned gene expression polymerase chain reaction conventional and automated DNA sequencing site-directed mutagenesis and more! Elucidating the material with over 2250 edifying references, equations, drawings, and photographs, this state-of-the-art resource is a valuable hands-on guide for molecular and cell biologists, biochemists, bioprocess technologists, applied and industrial microbiologists, virologists, geneticists, chemical engineers, and upper-level undergraduate and graduate students in these disciplines.

Recombinant Gene Expression

Author: Paulina Balbás
Publisher: Springer Science & Business Media
ISBN: 9781592597741
Size: 13.54 MB
Format: PDF, Mobi
View: 69

Leading investigators, all of them experts on particular gene expression systems, provide a stellar set of original protocols for gene expression supported by overviews and troubleshooting guides for the biological systmes addressed. This book provides a global view of gene expression in prokaryotes, fungi, plants and animals and will be an invaluable reference in the collection of all biological science laboratories.

Production Of Recombinant Proteins

Author: Gerd Gellissen
Publisher: John Wiley & Sons
ISBN: 9783527604418
Size: 19.82 MB
Format: PDF, Kindle
View: 43

While the choices of microbial and eukaryotic expression systems for production of recombinant proteins are many, most researchers in academic and industrial settings do not have ready access to pertinent biological and technical information since it is normally scattered throughout the scientific literature. This book closes the gap by providing information on the general biology of the host organism, a description of the expression platform, a methodological section -- with strains, genetic elements, vectors and special methods, where applicable -- as well as examples of proteins produced with the respective platform. The systems thus described are well balanced by the inclusion of three prokaryotes (two Gram-negatives and one Gram-positive), four yeasts, two filamentous fungi and two higher eukaryotic cell systems -- mammalian and plant cells. Throughout, the book provides valuable practical and theoretical information on the criteria and schemes for selecting the appropriate expression platform, the possibility and practicality of a universal expression vector, and on comparative industrial-scale fermentation, with the production of a recombinant Hepatitis B vaccine chosen as an industrial example. With a foreword by Herbert P. Schweizer, Colorado State University, USA: "As a whole, this book is a valuable and overdue resource for a varied audience. It is a practical guide for academic and industrial researchers who are confronted with the design of the most suitable expression platform for their favorite protein for technical or pharmaceutical purposes. In addition, the book is also a valuable study resource for professors and students in the fields of applied biology and biotechnology."